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Abstract

Objective:To amplify human PHPT1 gene, and construct the C-terminal GFP fused vectors, investigate the expression and location of the fusion proteins in cells, as well as the relationship with lamellipodia. Methods:The cDNA sequence of PHPT1 gene was amplified and subcloned into pEGFP-N2 vectors. Transfecting of those vectors into HeLa cells to investigating the location statue of PHPT1 using Laser scanning confocal microscope, and further studied the relationship of PHPT1 location with lamellipodia formation. Results:The pEGFP-N2-PHPT1 prokaryotic expression vectors was constructed successfully and the location of PHPT1 in lamellipodia was closely related to lamellipodia formation. Conclusion:C-terminal GFP fused PHPT1 were expressed in the nucleus and the cytoplasm, especially in the leading edge of lamellipodia, and this location was closely related to lamellipodia formation and cell mobility.

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